【摘要】 利用茚三酮比色法测定出牛肉中游离氨基酸含量。对游离氨基酸的提取方法及测定体系的pH、最大吸收波长、反应时间、显色剂用量等进行了研究,并提出了最佳提取方法为蒸馏水煮制提取法,得出最佳的测定条件为pH值为6.8,沸水浴加热15min并冷却15min后加入KIO3稀释液,在最大吸收波长为568nm处进行测定。更多还原

【Abstract】 An approach to determinating the free-amino acid level of bovine meat with Ninhydrin colorimetric method has been studied in this paper. The extraction method of free-amino acid and experimental condition of Ninhydrin colorimetriy such as pH,maximum absorption wave-length,reaction time,color reagent have been discussed on bovine meat. The optimal extraction method was poached in distilled water and optimal experimental condition was pH=6.8,heated 10min in boil water,then cooled 15min and added KIO3 diluent and finally determined at 568 nm.更多还原