【摘要】 目的探讨EB病毒(EBV)感染与淋巴瘤基因组不平衡改变、临床表现、病理类型、疗效和预后的关系。方法免疫组化法检测EBV潜伏膜蛋白(LMP-1),原位杂交方法检测EBV编码的小RNA(EBERs)表达情况,比较基因组杂交(CGH)进行基因组水平不平衡改变的研究。结果EBV感染的霍奇金淋巴瘤(HL)和胃黏膜相关淋巴瘤(MALT)型基因组无不平衡改变。外周T淋巴细胞非霍奇金淋巴瘤(NHL)表现为基因异常的数目多,核型复杂,预后不良。大B细胞NHL、小B细胞NHL表现为基因组水平缺失与增益的数目少、化疗效果好。结论EBV感染后的NHL宿主细胞基因组水平改变,扩增与/或缺失数目多,临床表现呈进展性,化疗效果差,预后不良。更多还原

【Abstract】 Objection To study the correlation with Epstein-Barr virus(EBV) and lymphoma genomic imbalanced expression, manifestation, pathologic subtype and prognose. Methods EBV was detected by latent membrane protein(LMP-1) and EBERs . EBV positive patient’s genomic DNA were analysed by comparative genomic hybridiation (CGH) to investigate genome changes. Results There is no genomic imbanlance in EBV (+) HL and gastric MALToma. In peripheral T cell NHL, there are a number of genomic abnormal and complex karytype which are related with bad prognose. In small B cell NHL and large B cell NHL, there is little genomic delation or gains which are related with sensitivity to chemotherapy and good prognose. Conclution EBV infection in lymphoma is correlated with different pathologic subtype, genomic imbanlance, sensitivity to medicine and prognose. The more genomic delation or gains in NHL, the more aggressive in NHL.更多还原