【摘要】 基于细菌16 S rRNA和(或)23 S rRNA序列设计引物,利用PCR的方法鉴定出了奶牛隐性乳房炎的主要病原菌,包括金色葡萄球菌、大肠杆菌和无乳链球菌,避免了繁琐的培养过程,表明PCR技术是细菌鉴定非常有效、特异性高而又快速的方法,对隐乳的诊断很有价值。更多还原

【Abstract】 Absract:Primers were designed based on the DNA sequences of bacterium 16 S rRNA and(or) 23 SrRNA.This article presented a PCR detecting the major pathogens in subclinic mastitis,includingStaphylococcus aoreus,Escherichia coli,Streptococcus agalactiae.The method avoided the redundant cellculturing process,therefore,the result showed that PCR technology is an effective,specific and rapidmolecular tool to identify bacteria,and also a valuable method to diagnose bovine subclinic mastitis.更多还原