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青蒿琥酯影响L929和Colon26肿瘤细胞免疫抑制的体外研究

The Study of Effects of Artesunate on Immunosuppression from L 929 and Colon 26 Tumor Cells in Vitro

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【作者】 崔澄胡建军张拥军李保红王润田刘景东佟慧

【Author】 CUI Cheng,HU Jianjun,ZHANG Yongjun,et al.*Bethune Military Medical College,Shijiazhuang 050081,China

【机构】 石家庄白求恩军医学院石家庄白求恩军医学院石家庄河北医科大学基础所免疫室

【摘要】 目的研究青蒿琥酯对L929和Colon26肿瘤细胞培养上清免疫抑制作用的影响。方法制备体外经青蒿琥酯作用后,彻底洗去青蒿琥酯,再以新鲜培养液培养L929及Colon26,收取再培养上清,同时以不经青蒿琥酯作用的L929及Colon26同步处理的相应上清作对照。实验研究这些上清对小鼠脾细胞MTT法测定的NK杀伤和ConA诱导转化,以及流式细胞计数分析的CD4+及CD8+表达的影响。结果与对照组上清相比,青蒿琥酯作用L929和Colon26后,其第一次(AL1、AC1)及第二次48h再培养上清(AL2、AC2)对ConA诱导小鼠脾细胞转化的抑制率均明显降低(分别为37.50%±6.58%,P<0.01;27.83%±5.50%,P<0.05;48.71%±3.52%,P<0.01;5.48%±1.22%,P<0.01);CD4+CD8-细胞表达百分率均明显升高(分别为15.30%±3.51%,P<0.01;27.68%±2.78%,P<0.05;10.69%±3.42%,P<0.01;28.02%±1.08%,P<0.01);AL1、AL2及AC1上清作用后,NK杀伤活性均明显升高(杀伤率分别为67.58%±4.83%,P<0.01;66.14%±4.10%,P<0.01;57.21%±3.38%,P<0.05),AC2对NK杀伤未产生明显改变(杀伤率为45.42%±8.98%,P>0.05)。结论青蒿琥酯可下调L929及Colon26肿瘤细胞培养上清的免疫抑制,下调肿瘤免疫抑制可能是青蒿琥酯的抗肿瘤新机制之一。

【Abstract】 Objective To study the effects of artesunate on immunosuppression caused with L 929 and Colon 26 tumor cells in vitro.Methods Re-cultured supernatants of L 929 and Colon 26 after treated by artesunate and followed by being washed respectively, and the corresponding supernatants of L 929 and Colon 26 treated without artesunate were collected as control.Through MTT,the effects of different supernatants on transformation induced by Con A and NK activity of murine splenocytes were studied.And through flow cytometry assay,the effects of different supernatants on murine splenocytes’ expression of CD 4+ and CD 8+ were analyzed.Results Compared with control supernatants,the first (A-L 1,A-C 1) and second re-cultured supernatants of L 929 and Colon 26 (A-L 2,A-C 2) decreased the inhibitory rates of murine splenocytes’ transformation induced by Con A significantly (37.50%±6.58%,P<0.01;27.83%±5.50%,P<0.05;48.71%±3.52%,P<0.01;5.48%±1.22%,P<0.01,respectively),and increased the proportion of CD 4+CD 8- cells greatly (15.30%±3.51%,P<0.01;27.68%±2.78%,P<0.05;10.69%±3.42%,P<0.01;28.02%±1.08%,P<0.01,respectively);A-L 1,A-L 2 and A-C 1 but not A-C 2 up-regulated NK killing activity obviously (killing rate were 67.58%±4.83%,P<0.01;66.14%±4.10%,P<0.01;57.21%±3.38%,P<0.05;45.42%±8.98%,P>0.05,respectively).Conclusion Artesunate can exert down-regulating effects on the immunosuppression from L 929 and Colon 26 tumor cells.Down-regulating the immunosuppression may be one of new anti-tumor mechanisms of artesunate.

【关键词】 青蒿琥酯L929Colon26免疫抑制
【Key words】 ArtesunateL 929Colon 26Immunosuppression
  • 【文献出处】 白求恩军医学院学报 ,Journal of Bethune Military Medical College , 编辑部邮箱 ,2006年03期
  • 【分类号】R73-3
  • 【被引频次】4
  • 【下载频次】98
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