【摘要】 目的研究青蒿琥酯对L929和Colon26肿瘤细胞培养上清免疫抑制作用的影响。方法制备体外经青蒿琥酯作用后,彻底洗去青蒿琥酯,再以新鲜培养液培养L929及Colon26,收取再培养上清,同时以不经青蒿琥酯作用的L929及Colon26同步处理的相应上清作对照。实验研究这些上清对小鼠脾细胞MTT法测定的NK杀伤和ConA诱导转化,以及流式细胞计数分析的CD4+及CD8+表达的影响。结果与对照组上清相比,青蒿琥酯作用L929和Colon26后,其第一次(AL1、AC1)及第二次48h再培养上清(AL2、AC2)对ConA诱导小鼠脾细胞转化的抑制率均明显降低(分别为37.50%±6.58%,P<0.01;27.83%±5.50%,P<0.05;48.71%±3.52%,P<0.01;5.48%±1.22%,P<0.01);CD4+CD8-细胞表达百分率均明显升高(分别为15.30%±3.51%,P<0.01;27.68%±2.78%,P<0.05;10.69%±3.42%,P<0.01;28.02%±1.08%,P<0.01);AL1、AL2及AC1上清作用后,NK杀伤活性均明显升高(杀伤率分别为67.58%±4.83%,P<0.01;66.14%±4.10%,P<0.01;57.21%±3.38%,P<0.05),AC2对NK杀伤未产生明显改变(杀伤率为45.42%±8.98%,P>0.05)。结论青蒿琥酯可下调L929及Colon26肿瘤细胞培养上清的免疫抑制,下调肿瘤免疫抑制可能是青蒿琥酯的抗肿瘤新机制之一。更多还原

【Abstract】 Objective To study the effects of artesunate on immunosuppression caused with L 929 and Colon 26 tumor cells in vitro.Methods Re-cultured supernatants of L 929 and Colon 26 after treated by artesunate and followed by being washed respectively, and the corresponding supernatants of L 929 and Colon 26 treated without artesunate were collected as control.Through MTT,the effects of different supernatants on transformation induced by Con A and NK activity of murine splenocytes were studied.And through flow cytometry assay,the effects of different supernatants on murine splenocytes’ expression of CD 4+ and CD 8+ were analyzed.Results Compared with control supernatants,the first (A-L 1,A-C 1) and second re-cultured supernatants of L 929 and Colon 26 (A-L 2,A-C 2) decreased the inhibitory rates of murine splenocytes’ transformation induced by Con A significantly (37.50%±6.58%,P<0.01;27.83%±5.50%,P<0.05;48.71%±3.52%,P<0.01;5.48%±1.22%,P<0.01,respectively),and increased the proportion of CD 4+CD 8- cells greatly (15.30%±3.51%,P<0.01;27.68%±2.78%,P<0.05;10.69%±3.42%,P<0.01;28.02%±1.08%,P<0.01,respectively);A-L 1,A-L 2 and A-C 1 but not A-C 2 up-regulated NK killing activity obviously (killing rate were 67.58%±4.83%,P<0.01;66.14%±4.10%,P<0.01;57.21%±3.38%,P<0.05;45.42%±8.98%,P>0.05,respectively).Conclusion Artesunate can exert down-regulating effects on the immunosuppression from L 929 and Colon 26 tumor cells.Down-regulating the immunosuppression may be one of new anti-tumor mechanisms of artesunate.更多还原