【摘要】 目的:研究山葡萄多酚(PVAR)抗衰老、抗氧化作用。方法:选用老龄(20月龄)Wistar雄性大鼠40只,采用区间随机分组方法按体重均分为4组,即老龄阴性对照组(A组),喂基础饲料;2个老龄PVAR组(B、C组),分别喂含200、400mg·kg-1·d-1PVAR的基础饲料;老龄阳性对照组(D组),喂含200mg·kg·d-1蜂花粉的基础饲料。连续饲养8周后,测定大鼠红细胞膜、血浆及肝脏MDA含量、红细胞膜巯基含量、唾液酸含量、Na+-K+-ATP酶活性和红细胞膜流动性、血浆GSH-Px活性。结果:与A组比较,B、C组和D组大鼠组织MDA含量显著降低(P<0·05或P<0·01);C组及D组红细胞膜巯基含量、唾液酸含量、红细胞膜Na+-K+-ATP酶活性及血浆GSH-Px活性显著升高(P<0·05)。B、C组与D组各项指标比较差异均无显著性(P>0·05)。结论:PVAR对老龄大鼠具有抗衰老、抗氧化作用。更多还原

【Abstract】 Objective To study the anti-ageing and antioxidation of polyphends of vitis amurensis Rupr(PVAR).Methods Forty senile male Wistar rats (20 months) were divided into 4 goups according rats′ weights:senile negtive control group(A), fed with basic forage;two senile PVAR groups(B and C),fed with basic forage inchuding 200 and 400 mg·kg~ -1 ·d~ -1 PVAR;senile positive control(D), fed with basic forage inchuding 200 mg·kg~ -1 ·d~ -1 melissic powder.After fed continuously for 8 weeks,the MDA contents in RBC-membrane, plasma and liver,sulfhydryl content in RBC-membrane, sialic acid content,activity of Na~+-K~+-ATPasc, fluidity of RBC-membrane,and activity of plasma GSH-Px in rats were detected.Results Compared with group A,the contents of MDA in groups B,C,and D decreased significantly(P<0.05,P<0.01);sulfhydryl contents in RBC-membrane and activities of plasma GSH-Px in group C and D increased significantly(P<0.05).There were no significant differences of various indexes between group B,C and group D (P<0.05).Conclusion PVAR has anti-ageing and antioxidation on senile rats.更多还原