【摘要】 目的:探讨多次小剂量pEgr-IFN-γEndostatin基因-放射治疗对荷Lewis肺癌小鼠的抑瘤效应。方法:将荷瘤小鼠随机分为对照组、10 Gy照射组、4次2.5 Gy照射组、4次质粒组、质粒+10 Gy组和4次(质粒+2.5 Gy)组。小鼠肿瘤局部注射脂质体包裹的质粒后36 h,接受X射线照射,观察各组小鼠治疗后不同时间肿瘤生长速率,18 d后计数各组小鼠肺转移结节数,并称量瘤重。结果:治疗后12~18 d,4次(质粒+2.5 Gy)组肿瘤生长速率明显低于质粒+10 Gy组(P<0.001)。治疗后18 d,4次(质粒+2.5 Gy)组小鼠肺转移结节数和瘤重明显低于质粒+10 Gy组(分别为P<0.001和P<0.01)。结论:多次小剂量pEgr-IFNγ-Endostatin基因-放射治疗的抑瘤效应优于单次大剂量基因-放射治疗。更多还原

【Abstract】 Objective To study the anti-tumor effect of repeated low dose pEgr-IFNγ-Endostatin gene-radiotherapy on mice bearing Lewis lung carcinoma. Methods Mice bearing Lewis lung carcinoma were divided randomly into control,10 Gy,4×2.5 Gy,4×P,P + 10 Gy and 4×(P + 2.5 Gy) group.The pEgr-IFNγ-Endostatin plasmid packaged with liposome was injected into tumors which were irradiated by X-ray 36 h later.Tumor growth rates at different time were observed after gene-radiotherapy.Number of lung metastatic nodes and tumor weight of the mice were detected 18 d after gene-radiotherapy.Results Tumor growth rate of the mice in gene-radiotherapy for four-time group was significantly lower than that for only once group 12-18 d after gene-radiotherapy.Number of lung metastatic nodes and tumor weight of the mice in gene-radiotherapy for four-time group were significantly lower than those for only once group 18 d after gene-radiotherapy.Conclusion Anti-tumor effect of repeated low dose pEgr-IFNγ-Endostatin gene-radiotherapy is better than that of high dose for only once.更多还原