【摘要】 目的:探讨5-羟色胺(5-HT)对胎盘滋养细胞凋亡的影响及其与妊娠高血压疾病发生、发展的相关关系。方法:将正常妊娠37~39周经剖宫产术获取的无菌胎盘滋养细胞分离、纯化、高糖型DMEM培养传代,第1~2代培养的细胞分成4组,加无菌蒸馏水对照组和加入0.1、1及10μmol.L-1浓度5-HT组,分别孵育培养3、6、12及24 h后,采用TdT末端转移酶介导的原位缺口末端标记法(TUNEL)检测各组培养的胎盘滋养细胞凋亡指数;采用电镜观察各组凋亡滋养细胞的超微结构。结果:10μmol.L-15-HT组培养3、6、12及24 h可使胎盘滋养细胞凋亡指数分别升至8.40±2.48、17.82±5.43、29.53±7.41及45.56±12.10,与对照组(1.21±0.97、2.30±1.24、3.89±2.32及7.34±5.59)比较,差异均有显著性(P<0.05);1μmol.L-15-HT组在培养12及24 h可使胎盘滋养细胞凋亡指数升高至17.96±3.48、23.76±8.47,与对照组比较差异均有显著性(P<0.05);0.1μmol.L-15-HT组胎盘滋养细胞凋亡指数与对照组比较,差异无显著性(P>0.05)。透射电镜下可见对照组及0.1、1和10μmol.L-1浓度5-HT处理组发生凋亡的胎盘滋养细胞均呈特征性的凋亡细胞改变:染色质固缩成块,核仁裂解消失,可见凋亡小体。结论:高浓度5-HT可促进胎盘滋养细胞凋亡,此机制可能与妊娠期高血压疾病的发生、发展有关。更多还原

【Abstract】 Objective To investigate the effects of 5-hydroxytryptamine（5-HT） on apoptosis of cultured human cytotrophoblast cells and its possible mechanism involved in pathogenesis of pregnancy-induced hypertension syndrome.Methods Human cytotrophoblast cells from three term pregnancy（37-39 weeks） women were cultured and divided into four groups: normal control group,5-HT groups(0.1,1 and 10 μmol·L^-1).The cells were cultivated for 3,6,12 and 24 h,respectively.Apoptotic index was measured by TdT-mediated biotinyated-dUTP nick end labeling（TUNEL） and morphological features of apoptotic cells were observed under electron microscope.Results When cells were cultivated for 3,6,12 and 24 h,compared with the normal control group（1.21±0.97,2.30±1.24,3.89±2.32 and 7.34±5.59,respectively）,apoptotic indexes of cytotrophoblast cells in(10 μmol·L^-1) 5-HT group were significantly increased（8.40±2.48,17.82±5.43,29.53±7.41,（45.56±）12.10,respectively）（P<0.05）;the apoptotic indexes in 1 μmol·L^-1 5-HT group were increased to 17.96±3.48 and 23.76±8.47（P<0.05） when cells were cultivated for 12 and 24 h;but there was no significant difference between 0.1 μmol·L^-1 5-HT group and normal control group（P>0.05）.The significant morphological changes of apoptotic cytotrophoblast cells in various groups were found under electron microscope.Apoptotic cytotrophoblast cells were obviously compact and the chromatins were formed as mass and apoptotic bodies were also found under electron microscope.Conclusion 5-HT can promote the apoptosis of human cytotrophoblast cells and its mechanism may be correlated with pregnancy induced-hypertension syndrome.更多还原