【摘要】 目的:探讨不同时间和不同浓度脂多糖(LPS)对小鼠巨噬细胞株J774A.1细胞CD14表达的影响。方法:用不同浓度LPS刺激J774A.1细胞后,FITC-CD14单克隆抗体染色45 min,用流式细胞术检测J774A.1细胞CD14的表达,观察用LPS刺激不同时间(1、2、4、8及16 h)后J774A.1细胞CD14表达的变化;选择最佳时间后,观察不同浓度(1、10、50、100、500及1 000μg.L-1)LPS对J774A.1细胞CD14表达的变化。结果:在时间-效应关系上,当LPS浓度为1μg.L-1时,刺激1、2及4 h后CD14表达增强(分别为23.80±5.07、23.04±2.88、28.22±1.54),与对照组(12.50±3.71)比较差异有显著性(P<0.01),LPS浓度为10μg.L-1时,刺激1和2 h后CD14表达增强(分别为40.85±6.05、26.63±6.17),与对照组(12.50±3.71)比较差异有显著性(P<0.05,P<0.01),而LPS浓度为50μg.L-1时,刺激1 h后CD14表达增强(47.40±2.85),与对照组(12.50±3.71)比较差异有显著性(P<0.01)。在剂量-效应关系上,刺激4 h时LPS浓度为小于10μg.L-1时CD14表达蛋白明显高于对照组(P<0.01)。结论:用LPS刺激J774A.1细胞表达CD14时,LPS刺激时间最好是在4 h以内,刺激浓度最好不要超过50μg.L-1。更多还原

【Abstract】 Objective To study the dose-and time-effect relationship between lipopolysaccharide（LPS） and CD14 expression in the cell line J774A.1 of mouse macrophages. Methods The cell line J774A.1 was stimulated with different doses of LPS,and the CD14 surface-positive cells from the cell line J774A.1 were detected by flow cytometry（FCM）45 min after stained with FITC-CD14 monoclonal antibody. The changes of CD14 surface expression in the cell line J774A.1 at different time（1,2,4,8 and 16 h）after stimulated with LPS were observed.After the best stimulant time was selected,the cell line J774A.1 was treated with different doses(1,10,50,100,500 and 1 000 μg·L^-1)of LPS and the changes of CD14 surface expression were observed.（Results In view） of time-effect,compared with control group（the positive rate was 12.50±3.71）,LPS significantly enhanced the CD14 expression at 1,2 and 4 h after stimulation（the positive rates were 23.80±5.07,（23.04±）2.88 and 28.22±1.54,respectively） when the dose of LPS was 1 μg·L^-1（P<0.01）.When the concentration of LPS was 10 μg·L^-1,CD14 expression strengthened at 1 and 2 h after stimulation（the positive rates were（40.85±）6.05 and（26.63±6.17,） respectively）（P<0.05,P<0.01）.LPS obviously stimulated CD14 expression at 1 h after stimulation（the positive rate was 47.40±2.85）when the dose of LPS was 50 μg·L¹（（P<0.01））.In the dose-effect,the stimulant effect of LPS occurred when LPS was administered only below the dose of 10 μg·L^-1 at 4 h after stimulation（P<0.01）. Conclusion LPS can significantly stimulate the expression of CD14 in the cell line J774A.1 below the dose of 50 μg·L^-1 within 4 h.更多还原