【摘要】 本文提出应用立体编织技术和快速成形技术分别制造支架内部结构的负型模具和外形结构的支撑模具,将多种生物材料和生长因子装配制备可降解生物仿生骨支架。设计25mm犬桡骨缺损修复实验,研究材料相同时,构造结构的支架(试验组)和均匀混合材料的支架(对照组)对骨修复的作用。结果显示两种支架均具有良好的生物相容性、骨传导和骨诱导作用。构造结构的支架明显促进新骨形成,材料内外同时降解,并引导新生组织首先在设计管道内成骨。分析认为该方法在复合多种材料时不需要高温烧结,不产热、可塑性极好,不改变材料和生长因子的性质,连通的管道保证了短时间内不同的生物材料对新骨形成作用同时存在,相互促进。更多还原

【Abstract】 A novel fabrication of degradable scaffold is presented by using several biomaterials and growth factor(GF).Chitosan fibers can be braided into the internal fiber microstructure of the scaffold by a three-dimensional braiding(3DB) method,and rapid prototype & rapid tools(RP/RT) techniques can be used to rapidly fabricate the outer shape mold of the scaffold.A kind of scaffold was fabricated by the novel method in this paper as a test,but another was fabricated by mixing the same biomaterials as a control.These two kinds of scaffolds were implanted into the 25mm canine radius defect respectively in order to investigate their osteoconductivity.The results demonstrated that both scaffolds had good biocompatibility,bone induction and bone conduction.The scaffolds with 3D fiber architecture had obviously accelerated bone regeneration in comparison with the control;in detail,the materials biodegraded inside and outside and the bone ingrowth first occurred along the chitosan fibers in the scaffold.In summary,the method in this paper not only can be easily shaped but also can not change the bio-properties of materials and GF since it does not need hightemperature sintering and not produce heat,so the actions of the several biomaterials for bone regeneration interacted simultaneously through the interconnective macrochannels in the in-vivo scaffolds,which left by the biodegradation of the internal fiber.更多还原