【摘要】 考察了研磨法、有机溶剂法和超声波破碎法对根瘤土壤杆菌细胞破碎的影响。结果表明,在有机溶剂破碎条件下,最佳搅拌时间为3.5 h,此时CoQ10的含量为5.703 mg/L;由正交实验分析得出,菌体浓度为40 mg/mL,作用时间为12 min,破碎功率为250 W时,超声波破碎的效果最佳;与上述两种常规破碎方法比较,在研磨的条件下,加入抗氧化剂BHT(2,6-二叔丁基对甲酚)的细胞破碎效果最好,CoQ10的含量为8.012 mg/L,比不加抗氧化剂BHT研磨时提高了约38%,抗氧化剂BHT的最佳质量分数为20%。更多还原

【Abstract】 In this paper,the effects of milling,disruption with organic solvent and ultrasonic on disruption of Agrobacterium tumefaciens KY-3085（ATCC 4452） Strain were compared.The results showed that the yield of CoQ₁₀ was 5.703mg/L when mixing time was 3.5h,on the condition of disruption with organic solvent.The orthogonal experiment by ultrasonic cell disruption indicated the optimum conditions were 40mg/mL concentration,12min,250W.Compared with above,milling with quartz and acetone was the best when the antioxidant BHT（2,6-Di-tert-butyl-4-methylphenol） was added.In this method,the yield of CoQ₁₀ was 8.012mg/L,increased of 38 percent.The optimizated addition of antioxidant BHT was 20%.更多还原