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不同GO基因植物表达载体对番茄遗传转化效率的研究

Transformation Rate of Two Plant Expression Vector pCPGO and pCBGO with GO Gene in Tomato

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【作者】 王全华李素梅尹国香曹守军张焕春李云

【Author】 WANG Quan-hua~1,LI Su-mei~1,YIN Guo-xiang~1,CAO Shou-jun~1,ZHANG Huan-chun~1,LI Yun~2(1.Yantai Academy of Agricultural Sciences,Shandong,265500;2.Yantai Fushan District Vegetable Office,Shandong,265500)

【机构】 山东省烟台市农科院蔬菜所山东省烟台市福山区蔬菜办 265500265500

【摘要】 通过PPT质量浓度的梯度试验,筛选出番茄子叶出愈、芽分化和再生植株生根的最适宜PPT浓度均为1.0 mg/L。pCPGO和pCBGO两个植物表达载体转化8个番茄自交系材料的试验结果表明,不同番茄自交系对这两种表达载体的遗传转化效率存在较大的差异;相同的载体转化不同的番茄自交系,其遗传转化率也存在较大的差异;同一番茄品系,由于转化载体不同,遗传转化率也不同。pCPGO和pCBGO转化率最高的分别为Pr5 76.47%和Pr1 72.4%;而低的分别仅为Pr3 13.89%和Pr4 2.7%。

【Abstract】 Four concentration of PPT was tested in tomato.Results showed the optimistic PPT concentration of calus,shoot formation and rootage of tomato explants were 1.0 mg/L.Eight tomato lines were transformed using plant expression vector pCPGO and pCBGO.The results demonstrated transformation frequency of various tomato lines was roughly different in two expression vector.That was in same vector and transformation frequency of same tomato line duing to various plant expression vector.The highest transformation frequency of pCPGO and pCBGO in all lines were Pr5,76.47% and Pr1,72.4%,the lowest of those were Pr3,13.89% and Pr4,2.7%.

【关键词】 番茄GO植物表达载体遗传转化
【Key words】 TomatoGO plant expression vectorTransformation
【基金】 山东省良种产业化开发项目资助,项目号为鲁科字[2001]500号
  • 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2006年06期
  • 【分类号】S641.2
  • 【被引频次】2
  • 【下载频次】104
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