【摘要】 采用体外细胞培养技术,对南海7种海藻多糖体外抗单纯疱疹Ⅰ型病毒(HSV-1)和柯萨奇B组3型病毒(CoxB3)活性进行研究。7种海藻多糖均表现出良好的抗病毒活性。其中,孔石莼多糖、蜈蚣藻多糖可明显抑制HSV-1,细胞病变半数抑制浓度(IC50)均为3.90μg/mL,选择指数(SI)分别达363和296.4。孔石莼多糖、小石花菜多糖可明显抑制CoxB3,IC50分别为1.95μg/mL和7.81μg/mL,SI分别为>1 025.6和212。同时理化实验结果表明,多糖样品均为硫酸酯多糖,其硫酸基和岩藻糖的含量与多糖的抗病毒效果密切相关。更多还原

【Abstract】 The antiviral activities of polysaccharides from seven kinds of algae collected from the South China Sea against herpes simplex virus type 1（HSV-1） and coxsackievirus（CoxB₃） in vitro were reported in this paper.The results indicated that these polysaccharides all possessed good antiviral effects.The polysaccharides from Ulva pertusa Kjellm and Grateloupia filicina（L.） C.Ag inhibited HSV-1 at the same IC₅₀ values of 3.90μg/mL,and SI values of 363 and 296.4 respectively.The polysaccharides from Ulva pertusa Kjellm and Gelidium divaricatum Martens inhibited CoxB₃ at the IC₅₀ values of 1.95μg/mL and 7.81μg/mL,and SI values of >1025.6 and 212 respectively.The physicochemical experiments showed that these seven polysaccharides all contained sulfate groups,the sulfate groups and fucose composition in the polysaccharides were closely related to their antiviral action.更多还原