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转染人乳头瘤病毒16型E6E7重组基因及感染模型细胞建立
Transfection of Human Papillomavirus Type 16-E6E7 Gene and Establishment of an Infected Cell Line Model
【Abstract】 To investigate the functions of HPV-16 E6E7 protein in the development of cervical cancer,a recombinant plasmid pEGFP-HPV16E6E7,containing green fluorescent protein and HPV-16 E6E7 proteins was constructed and was transfected into HPV-negative cell line C33A mediated by liposome.The efficiency of tranfection was determined by inverted fluorescent microscope and the expression of E6E7 was detected by Western blot.It showed that transfection of C33A cell and Caski cell by HPV-16 E6E7 gene established a pair of transfected cell types to study the carcinogenesis of E6 and E7 proteins.
【关键词】 人乳头病毒16型E6E7基因；
基因转染；
基因表达；
【Key words】 human papillomavirus type 16 E6E7 gene； gene transfer； gene expression；
【Key words】 human papillomavirus type 16 E6E7 gene； gene transfer； gene expression；
【基金】 国家自然科学基金(30271358);国家重点基础研究发展规划项目(973项目,2002CB513100)
- 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,2006年02期
- 【分类号】R373
- 【下载频次】161