【摘要】 目的探讨外周血单个核细胞(PBMCs)中乙型肝炎病毒(HBV)X、P基因整合与原发性肝细胞癌(HCC)的关系。方法分离HBsAg阳性的40例原发性肝癌病人PBMCs,提取基因组DNA;分别设计HBV X基因、P基因PCR引物,检测HCC病人PBMCs基因组中HBV的X基因和P基因的整合情况。结果HCC病人X基因在PBMCs中的整合率为77.5%(31/40),P基因整合率为15.0%(6/40),二者差异有显著性(χ2=31.43,P<0.001)。结论PBMCs中存在HBV X、P基因的整合,X基因片段的整合及表达是HCC发生的主要因素。更多还原

【Abstract】 ObjectiveTo investigate the relationship between primary liver cancer(PLC) and integration of HBV X gene and P gene in peripheral blood mononuclear cells(PBMCs) in PLC patients.MethodsPrimers X1,X2 were designed for HBV X Gene and P1,P2 for P gene.Extracted DNA from isolated PBMCs in 40 PLC patients with HBsAg positive.PCR was used to determine the integration of HBV P and X gene.ResultsThe integration of X gene and P gene was 77.5%(31/40) and 15.0%(6/40) respectively.The difference was significant(χ~2=31.43,P<0.001).ConclusionThe integration of HBV X and P exists in peripheral blood mononuclear cells,and the expression of X gene plays a major role in the occurrence of primary liver cancer.更多还原