【摘要】 目的建立用高效液相色谱法(highperformanceliquidchromatography,HPLC)同时测定复方维生素注射液(4)中三种组分含量的方法。方法采用HPLC法,C18色谱柱(150mm×4.6mm,5μm),流动相甲醇-乙醇(80∶20),检测波长270nm。结果维生素A棕榈酸酯在12.5~125.5μg/ml之间线性良好(r=0.9999),平均回收率为99.38%,相对标准偏差(RSD)为1.36%;维生素E在125~1251μg/ml之间线性良好(r=0.9999),平均回收率为98.87%,RSD为0.95%;维生素K1在16.5~166.5μg/ml之间线性良好(r=0.9999),平均回收率为98.08%,RSD为1.57%。结论HPLC法测定复方维生素注射液(4)中3种维生素的含量专属性强,灵敏度高,准确性好,可用于复方维生素注射液(4)的含量测定。更多还原

【Abstract】 Objective:To determine the three contents in compound four vitmine injection by high performance liquid chromatography（HPLC）.Methods:HPLC was used.C18 column（250 mm×4.6 mm,5 μm） was selected,the mobile phase was methanol-alcohol（80∶20）.Detection was performed by UV with wavelength of 270 nm.Results:A linearity of retinyl palmitate was obtained from 12.5 to 125.5 μg/ml with good correlation（r=0.999 9）.The average recovery of retinyl palmitate was 99.38%,and RSD was 1.36%.A linearity of vitamin E was obtained from 125 to 1251 μg/ml with good correlation（r=0.999 9）.The average recovery of vitamin E was 98.87%,and RSD was 0.95%.A linearity of vitamin K₁ was obtained from 16.5 to 166.5 μg/ml with good correlation（r= 0.999 9 ）.The average recovery of vitamin K₁ was 98.08%,and RSD was 1.57%.Conclusions:The RP-HPLC is sensitive and accurate in determining the three contents in compound four vitamine injection.It can be applied efficiently.更多还原