【摘要】 研究了以尾叶桉(Eucalyptusurophylla)优树(U6无性系)无菌苗的叶子和茎段作为外植体诱导愈伤组织、丛生芽发生以及植株再生的过程。通过多种生长调节剂不同浓度组合的对比试验,确定了U6快繁体系的最适宜培养条件:(1)愈伤组织诱导培养基:MS+1-2mg/L2,4-D;(2)芽增殖培养基:MS+0.5mg/L6-BA;(3)生根培养基:1/2MS+2.0mg/LNAA。更多还原

【Abstract】 Callus induction,shoot organogenesis and plantlet regeneration from leaf and sprout explants of E.urophylla’s asepsis seedling were studied in the paper. The optimal culture conditions were established by the comparative experiments of different concentration combinations of many growth regulators as follows: (1) callus induction media: MS+1-2mg/L 2,4-D; (2) shoot multiplication culture medium: MS+0.5mg/L 6-BA; (3) rooting culture medium: 1/2 MS+2.0mg/L NAA.更多还原