【摘要】 通过扫描电子显微镜、能量分析谱、红外光谱和悬浮酵母菌的动电电位等分析手段,研究了悬浮酵母菌对Hg(Ⅱ)的吸附机理。由扫描电镜图像及能量分析谱可以看出,悬浮酵母菌吸附Hg(Ⅱ)后形态发生变化,悬浮酵母菌对Hg(Ⅱ)的吸附存在离子交换吸附;动电电位及pH曲线分析表明,悬浮酵母菌的零电点为pH=3.7,pH值是影响吸附效果的重要因素,溶液pH值为4.33时,吸附效果最好,去除率为99.95%;溶液pH<4.0时,静电作用为主要的吸附机理;从吸附等温线可以得出,在溶液中Hg(Ⅱ)的初始质量浓度为601.8 mg/L,菌质量浓度为20 g/L时,吸附达到饱和,此时的负载量为10.24 mg/g;红外光谱分析表明,悬浮酵母菌对Hg(Ⅱ)的主要吸附位点为—OH、—NH2、C=O、P=O和S=O。更多还原

【Abstract】 The present paper is to introduce the authors’ research into the mechanism of Hg（Ⅱ） biosorption by saccharomyces cerevisiae while applying modern analysis means,such as SME,electron spectrum,FT-IR analysis,and zeta potential apparatus.The contrasted results of SME and electron spectrum show that the form of saccharomyces cerevisiae has changed as the result of the biosorption on Hg²⁺.The other findings of our research can be stated as follows.（1） An obvious hydronium commutation in the course of saccharomyces cerevisiae biosorption on Hg²⁺;（2） The isoelectric point of saccharomyces cerevisiae proves at pH=3.7 as the result of zeta potential and pH curve’s analysis;（3） As an important affecting factor,the pH value is 4.33;（4） When the value of pH is under 4.33,i.e.on the ideal adsorbing condition,the removal rate can reach 99.95%,with the biosorption of Hg（Ⅱ） by saccharomyces cerevisiae being the main static biosorption. From the absorbing isotherm,it can be found that,when the initial concentration of Hg solution is 601.8 mg/L,the concentration of the germ will be 20 g/L,with the biosorption reaching saturation,and the loading quantity will be 10.24 mg/g.Thus,the FT-IR analyses indicate that the mainly adsorbing positions of saccharomyces cerevisiae to Hg（Ⅱ） are —OH,—NH₂,C=O,P=O,and S=O.更多还原