【摘要】 采用PCR技术扩增出小尾寒羊催乳素基因5′侧翼调控区的161 bp大小的片段,经SSCP检测到2种基因型(AA、AB),将这2种基因型片段分别克隆到pGEM-T Easy质粒中,重组质粒用PCR扩增进行阳性克隆鉴定,然后测定核苷酸序列。催乳素基因扩增片段第63处发生了单碱基的改变(C→A)。小尾寒羊催乳素基因2种基因型核苷酸序列与母牛的同源性为92.5%。更多还原

【Abstract】 The 161 bp fragment of 5′flanking region of prolactin gene of Small Tail Han sheep was amplified successfully by PCR.Two genotypes(AA and AB) were detected by SSCP analysis and cloned into pGEM-T Easy vector.The positive clones were further identified by PCR amplification.The sequencing results showed that there was one single nucleotide mutation C→A at the site of 63 bp of amplified fragment of prolactin gene.The homology of nucleotide sequence of 5′flanking region of prolactin gene between Small Tail Han sheep and cow was 92.5%.更多还原