Phosphofructokinase-1(EC.2.7.1.11)gene from E.coli K-12 was amplified by PCR and inserted into plasmid pCMVT_NT~(TM) to create a reoombinant plasmid Ku-1.Then the plasmid was introduced into B4 and expressed.The pfkA gene was expressed in B44 strain \.The restraint of the whole passway of Glu metabolism was removed by increasing Phosphfructokinase-1.At the same time,the strain B44 showed respectively a glutamate productivity of 17.1% higher and a glutamatr/glucose conversion rate of 10.64% higher than those...
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