【摘要】 背景与目的:骨桥蛋白(osteopontin,OPN)是一种分泌性磷酸糖蛋白,可以同整合素和CD44v结合,在肿瘤发生、发展、侵袭、转移等病理过程中起作用。本研究探讨血浆中OPN检测,在预测肝细胞肝癌(hepatocellularcarcinoma,HCC)术后转移复发中的意义。方法:病例组选取手术切除的肝细胞癌患者56例,用ELISA法检测术前血浆OPN浓度,对照组取23例健康体检职工血浆标本。比较病例组与对照组血浆OPN水平;并随访病例组复发转移情况,分析血浆OPN水平与肿瘤转移复发的关系。结果:HCC组血浆OPN浓度为(864.4±391.9)!g/L,健康对照组血浆OPN浓度为(382.5±169.4)!g/L,两组相比较存在显著性差异(P<0.001)。随访期间,25例无复发,31例复发。肝细胞癌无复发组血浆OPN浓度为(744.6±323.9)!g/L,复发组血浆OPN浓度为(961.0±411.6)!g/L,复发组与无复发组血浆OPN浓度比较有显著性差异(P=0.039)。Cox模型多因素分析发现血浆OPN水平、肝硬化、肿瘤大小是预测肝癌转移复发的独立因素。结论:肝细胞癌患者血浆中OPN浓度可以作为预测肿瘤复发转移的潜在参考分子指标之一。更多还原

【Abstract】 BACKGROUND & OBJECTIVE: Osteopontin (OPN), a secretory phosphorylated glycoprotein and integrin-binding protein, has been involved in tumorigenesis, progression, invasion, and metastasis. OPN is over-expressed in a variety of human tumors. This study was to evaluate the value of OPN detection in forecasting the postoperative metastasis and recurrence of hepatocellular carcinoma (HCC). METHODS: Plasma OPN level of 56 patients with HCC was detected before operation by enzyme-linked immunosorbent assay (ELISA), and plasma samples of 23 healthy volunteers were also analyzed as control group. The correlation of plasma OPN level to metastasis and recurrence of HCC was analyzed. RESULTS: Plasma OPN level was significantly higher in HCC group than in control group [(864.4±391.9) ?滋g/L vs. (382.5±169.4) ?滋g/L, P<0.001]. During the follow-up, 25 patients had tumor recurred. Plasma OPN level was significantly higher in the patients with early HCC recurrence than in those without recurrence [(961.0±411.6) ?滋g/L vs. (744.6±323.9) ?滋g/L, P=0.039]. Cox multivariate analysis showed that plasma OPN level, cirrhosis, and tumor size were independent predictors for HCC recurrence and metastasis. CONCLUSION: Plasma OPN level may be a potential diagnostic marker for predicting metastasis and recurrence of HCC.更多还原