【摘要】 目的研究应用气相色谱-质谱联用仪测定羊肉中阿托品的方法,为此类检测提供依据。方法用酸性水萃取,经过滤处理,甲醇溶解,气相色谱-质谱联用仪测定;色谱条件:DB-5MS毛细管色谱柱,程序升温方式升温,起始温度1000C保持1m in,以150C/m in速率升温至2800C保持2m in;质谱条件:离子阱温度1500C,传输线温度2000C,歧管温度450C,电离方式EI(电子轰击),轰击能量70 eV,特征离子质量数42、82、124(M/Z)。结果本法最低检测限为20ng,平均回收率83%,相对标准差RSD11.6%,线性范围0~100ng。结论本方法适合于食物中毒样品中阿托品的测定。更多还原

【Abstract】 Objective In order to establish a method for determination of atropine in mutton.Methods The samples were purified by acidity water,dissolved by methyl alcohol,and then analyzed with GC-MS;The conditions for the GC were a DB-5MS capillary column,and the programming temperatures: initial temperatures were 100⁰C,hold 1min,rate of heating 15⁰C/min,from 100⁰C to 280⁰C,hold 2min;The conditions for the MS were that the temperature of the ion trap was 150⁰C,the temperature of the transfer line was 200⁰C,the temperature of the manifold was 45⁰C,the module option key was EI,the energy was 70eV,and the characteristic ions were 42、82 and 124（M/Z）.Results The detection limit of the method was 20ng,the recovery rate was 83%,and the RSD was 11.6%.The range of linearity was 0-100ng.Conclusions It was concluded that the method is suitable for determination of atropine in mutton.更多还原