【摘要】 对15个葡萄品种进行RAPD分析。从60条随机引物中筛选出10个多态性引物用于DNA扩增,共扩出71条DNA带,其中多态性DNA带47条,占总数的66.2%。通过聚类分析,分成4大类,美洲葡萄类群康可、奇妙无核自成一类,与欧亚种、欧美杂种分开;其中森田尼无核、无核白遗传关系近,最先聚在一起。该结果显示,欧亚类群遗传差异较大,具有丰富的遗传多样性,证明RAPD技术能在DNA分子水平上揭示葡萄遗传背景及亲缘关系。更多还原

【Abstract】 15 genotypes of grape(Vitis L) were analyzed with RAPD.Ten primers screened from 60 primers could amplify clearly and stably 71 bands.47 bands were examined for random amplified polymorphic DNA,which occupied 66.2%.The relationships among the 15 varieties based on their genetic distances were clustered with analysis method of the dendrogram.These varieties can be classified into 4 groups by cluster analysis,which V.labrusca L.was separated from V.vinifera L.and V.vinifera L× V.labrusca L.and Vitis Vinifera L.Centenial seedless and V.vinifera cv.Thompson seedless had a close genetic relationship,which was firstly clustered.The result indicated that V vinifera L.had a large difference on genetic distances and genetic diversity.It was proved that RAPD technique can successfully reveal the genetic variation and the genetic relationships of grape.更多还原