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薤白粗多糖提取工艺研究

Study on the Extraction Technology of Polysaccharide from Allium macrosttemon B.

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【作者】 夏新奎杨海霞李纯尹健

【Author】 XIA Xin-kui et al (Xinyang Agricultural College,Xinyang,Henan 464000)

【机构】 信阳农业高等专科学校生物工程系信阳农业高等专科学校生物工程系 河南信阳464000河南信阳464000

【摘要】 对中药薤白中可溶性粗多糖的提取工艺进行了研究。结果显示,影响多糖提取率因素的主次顺序为温度>提取时间>料液比。最佳提取工艺为提取温度90℃、料液比1∶12、提取时间90 min,薤白多糖的提取率为13.93%。同时研究了提取过程中脱蛋白的处理方法,结果显示,木瓜蛋白酶+Sevag法脱蛋白处理效果最佳。

【Abstract】 The extraction of polysaccharide from Allium macrosttemon B.was studied.With single factor test and orthogonal experiment design method we analyzed the influence of factors such as temperature(A),solid-liquid ratio(B) and time(C) on the extraction percent of the polysaccharides.The results indicated that the influence of the three factors on extraction percent of the polysaccharides was A>C>B and the temperature was the main factor.The optimum extraction technology was A3B2C3(temperature was 90?℃,solid-liquid ratio was 1∶12 and the extraction time was 90 min).Under these conditions,the extraction percent of soluble polysaccharides from Alluim macrosttemon B.was 13.93%.In addition,the method of removing protein was studied and the results indicated that Sevag method combined with the enzyme was best.

【基金】 河南省科技攻关项目(991020207)
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2006年17期
  • 【分类号】TQ461
  • 【被引频次】19
  • 【下载频次】336
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