【摘要】 湖北百合鳞片不同部位均可诱导出芽,其分化能力从强到弱依次为外层、中层、内层。0.8 cm×0.8 cm外植体的诱导率和污染率均高于0.5 cm×0.5 cm外植体。最佳芽诱导培养基是MS+6-BA 1.0 mg/L+NAA0.5 mg/L,其诱导率可达100%;最佳芽增殖培养基是MS+6-BA 0.2 mg/L+NAA 0.5 mg/L,相同浓度的NAA较2,4-D更有利于芽的增殖;最佳生根诱导培养基为1/2 MS+NAA 0.2 mg/L,含NAA的培养基诱导出的根短而粗壮,含IAA的培养基诱导出的根长且细,说明NAA诱导生根的效果要好于IAA。更多还原

【Abstract】 The bulblet of lilies can be induced from the different parts of Henryi L.,of which the ability from strong to weak is from the outer scales,the middle scales to the inner scales.The inducing rate and polluting rate of the 0.8?cm×0.8?cm in its size were higher than the 0.5?cm×0.5?cm.The best induction medium for scale culture was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L,of which the inducing rate was 100%.The best proliferation medium of shoots was MS+6-BA 0.2 mg/L+NAA 0.5 mg/L and compared with 2,4-D,the same concentration of NAA was more advantageous to the proliferation of bud.The best induction medium for roots was 1/2 MS+NAA 0.2 mg/L and the roots which were induced from the medium with NAA were short and robust;the roots which were induced from the medium with IAA were long,thin and week.We can see that NAA was better than IAA for rooting.更多还原