【摘要】 从豆天蛾幼虫虫体中分离纯化出一种新型的豆天蛾核型多角体病毒(CbNPV),提取基因组DNA,进行基因组测序。结果发现一个与杆状病毒F蛋白基因同源性很高的读码框序列,该长度为2 109 bp,编码702个氨基酸。氨基酸序列同源性分析表明:CbNPV F蛋白与II类NPV和I类NPV F蛋白的同源性分别为32.9%~61.8%和8.0%~16.1%,并且将它与多种I类NPV杆状病毒同功能蛋白GP64进行分析,同源性在9.1%~11.4%,表明CbNPV F蛋白与I类NPV的F及GP64蛋白的同源性很低,因此认为CbNPV属于II类NPV。更多还原

【Abstract】 Clanis bilineata Nuclear Polyhedrosis Virus(CbNPV)was purified from Clanis bilineata larva.To obtain the molecular information of the virus,the genomic DNA of CbNPV was extracted,and a DNA fragment library of the virus was constructed by Shotgun.The positive clones were sequenced and analyzed.A new open reading frame,which had high identificatiou with the F protein gene of most baculoviruses,was found in the library.The F protein gene of CbNPV was 2 109 bp long and encoded a protein with 702 amino acids.The characteristic and structure of the sequence was analyzed.The amino acid sequence analysis indicated that the CbNPV F protein had 8.0%～16.1%and 32.9%～61.8% identities with Group I and II NPVs F proteins,respectively.And then to be compared with many kinds of group I NPVs gp64 gene,the result indicated that they had 9.1%～11.4% homology,which showed that CbNPV belonged to Group II NPV.更多还原