【摘要】 建立了反相高效液相色谱法同时测定四数獐牙菜中的落干酸、獐牙菜苦苷、龙胆苦苷、芒果苷含量的方法。测定方法:在检测波长254 nm、柱温30℃、流速1 ml/min时,用ZORBAX SB-C18(250 mm×4.6 mm i.d.,5μm)柱,以甲醇和水(含0.04%H3PO4)在0～20min内由20%至35%线性梯度洗脱。试验结果:4种成分均达到基线分离,落干酸、獐牙菜苦苷、龙胆苦苷、芒果苷的线性范围分别为0.05～0.625μ(g r=0.999 9)、0.095～2.9μ(g r=0.999 8)、0.049～2.56μ(g r=0.999 7)、0.006～2.8μ(g r=0.999 9);回收率分别为101%(RSD=4.3%)、98.7%(RSD=3.3%)、99.5%(RSD=3.5%)、102%(RSD=1.0%)。结论:测定方法快速,结果准确、可靠。更多还原

【Abstract】 To establish a quantitative method of simultaneously determination of loganic acid,swertiamarin,gentiopicroside and mangiferin in Swertia tetraptera Maxim.by RP-HPLC.Method: The sample were separated on the column of Zorbax SB-C18(250 mm×4.6 mm i.d.,5 μm)which eluted with methanol and water(0.04 % phosphoric acid).The ratio of methanol and water increased from 20∶80 to 35∶65 in 20 min with detected wavelength at 254 nm,flow rate at 1 ml/min,column temperature at 30 ℃.Results: Four compounds were base-isolated.The linear ranges of loganic acid,swertiamarin,gentiopicroside and mangiferin were 0.05～0.625 μg(r=0.999 9),0.095～2.9 μg(r=0.999 8),0.049～2.56 μg(r=0.9997),0.006～2.8 μg(r=0.999 9)respectively,the average recoveries were 101 %(RSD=4.3 %),98.7 %(RSD=3.3 %),99.5 %(RSD=3.5 %),102 %(RSD=1.0 %)respectively.Conclusions: the method is rapid and precise.更多还原