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群体感应机制在生防细菌筛选中的应用
Isolation and Bio-control Effect of Bacterial Strain by Quorum Sensing Mechanism
【摘要】 根据群体感应机制,利用PCR技术,摸索出一套能够高效筛选生防细菌的方法。利用这一方法,从土壤中分离的1 399株细菌中筛出42株,占总分离株的3%,PDA培养基平板拮抗试验发现,其中有39株对香蕉尖孢镰刀菌有抑菌效果,占总筛选株数的95.2%。抑菌效果最好的分离株DFL059在田间试验中对香蕉枯萎病有较强的生防效果。
【Abstract】 Based on the Quorum sensing mechanism,an isolation model of bio-control of bacteria was designed with the method of PCR technique.42 strains were isolated from 1?399 strain bacteria.The percentage was 3% in total strain.On PDA medium,39 strains were found to suppress Fusarium oxysporum of banana in the 42 strains.The percentage was 95.2%.The DFL059 strain was found the best strain with the antagonistic ability to Fusarium oxysporum of banana.
【基金】 科技基础性工作和社会公益研究专项(2004DIB3J073)
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2006年07期
- 【分类号】S476
- 【被引频次】1
- 【下载频次】337