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棉花黄萎病拮抗菌发酵条件的初步研究
Studies on Fermentation Condition of Antagonistic Bacteria against Verticillium dahliae
【摘要】 本研究以棉花黄萎病拮抗菌DS45-2作为试验菌株,对抗菌物质发酵条件进行了初步摸索。6-12h的种子发酵液正处于对数生长期,最适于作种子液。在pH8.0的LBG培养基中30℃摇床恒温培养48h后其发酵液的抑菌效果最好。
【Abstract】 In this article,we cultured DS45-2 strain under different conditions and studied the antagonistic activities of the liquid ferment production.Experiments indicated that the optimum fermentation condition for DS45-2,an antagonistic bacteria against Verticillium wilt of cotton,are LBG medium of pH8.0,30℃,48h for rotational culture time.Under this condition,the strain could secrete lots of antifungal protein.
【关键词】 棉花黄萎病;
拮抗细菌;
发酵;
【Key words】 Verticillium wilt of cotton; antagonistic bacteria; fermentation;
【Key words】 Verticillium wilt of cotton; antagonistic bacteria; fermentation;
- 【文献出处】 安徽农学通报 ,Anhui Agricultural Science Bulletin , 编辑部邮箱 ,2006年11期
- 【分类号】S435.62
- 【被引频次】5
- 【下载频次】132