【摘要】 通过芦笋9个品种1.8 hm2大田实地调查,发现芦笋雄株中的雄性系两性株132株,有5个品种未发现雄性系两性株,玛丽华盛顿500W、Uc72各发现1株,西德全雄发现18株,鲁芦笋1号发现112株,并对发现的芦笋雄性系两性株进行目测和镜检观察,结果表明:应选择白色或黄白色的根尖,用1 mmol/L 8-羟基喹啉进行预处理5 h;解离用1 mol/L盐酸60℃恒温处理18 min效果最好;采用合理的压片方法,用双目显微镜可观察到二倍体和四倍体染色体。更多还原

【Abstract】 In this paper,9 asparagus varieties were investigated in 1.8 hm~2 field.The results showed that there were 132 andromonoecious asparagus plants including one Mary Washington plant,one Uc72 plant,18 West Germany plants,and 112 Lu Asparagus NO.1,and no andromonoecious plants were found in 5 varieties.At the same time,the found andromonoecious plants were observed by microscope,and the results indicated that white or yellow-white root tip should be choosed to pretreat 5 h in 1 mmol/L 8-hydroxy quinoline;it was optimum to dissociating 18 minutes at 60℃ constant temperature in 1 mmol/L HCl;the diploid and tetraploid chromosomes could be observed by binocular microscope with proper squash method.更多还原