【摘要】 背景与目的:研究已烯雌酚(diethylstilbestrol,DES)对新生ICR小鼠的致肿瘤作用,寻求建立DES致肺肿瘤模型经济有效的方法。材料与方法:ICR新生鼠,分设DES、乌拉坦(urethan,U)和U+DES组。U在14d以500mg/kg腹腔注射(intraperitoneal,ip);DES分别在1d、8d、15d ip,共3次,分别以注射日龄LD50的1/7、2/7、4/7为DES低(L)、DES中(M)、DES高(H)组。至26周时剖检,观察肿瘤发生情况。计算主要脏器系数、肺肿瘤发生率和平均肿瘤结节数。结果:新生鼠DES、U+DES及U各组均有明显的肺肿瘤发生。DES(L,M,H)各组的肺肿瘤发生率分别为16.7%、22.4%、43.1%;U+DES(L,M,H)各组分别为70.4%、90.9%、70.8%,U组为53.1%。U+DES(L,M)组肺肿瘤平均结节数高于DES(L,M)组,差异均有统计学意义(P<0.05)。结论:DES与U联合作用可致新生鼠肺肿瘤高发,可作为肺肿瘤模型建立的有效方法。更多还原

【Abstract】 BACKGROUND & AIM: In order to explore an economical and effective method for building lung tumor model induced by diethylstilbestrol(DES), we studied the carcinogenic effect on neonatal mice treated by DES. MATERIALS AND METHODS: The newborn mice were divided into DES, urethan(U) and U+ DES groups. U was given in 500 mg/kg dose ip on the 14th day after they were born. DES was administered by ip on 1 d, 8 d, 15 d in the doses of 1/7(L), 2/7 (M) and 4/7 (H) LD50 of the day of treated. At 26 weeks old ,they were sacrificed and checked for the formation of tumors. The organ index ,tumor incidence ratio and mean number of tumors were calculated. RESULTS: Lung tumors were obviously induced in tested neonatal mice. The incidence of lung tumor of DES(L,M,H) groups were 16.7% , 22.4% and 43.1% , the U+ DES(L,M,H)) were 70.4% ,90.9% and 70.8% respectively, and the U group was 53.1% . The mean numbers of lung tumors of U+ DES(L,M) were higher than the DES(L,M) respectively (P<0.05). CONCLUSION: High incidence of lung tumor could be induced by the combined action of DES and U in neonatal mice, which may be an useful and economical method to establish a lung tumor model.更多还原