【摘要】 背景与目的从鳞盖肉齿菌(Sarcodonscabrosuskarst)的二氯甲烷提取物中分离纯化得到SarcodoninG,对SarcodoninG进行抗肿瘤细胞增殖活性及其机制的研究。材料与方法利用四唑盐(MTT)比色法测定SarcodoninG对HeLa细胞增殖的抑制率;利用流式细胞术检测SarcodoninG对HeLa细胞的凋亡和P53蛋白表达的影响;利用电镜技术观察SarcodoninG对HeLa细胞形态学改变。结果SarcodoninG对体外培养的HeLa细胞的增殖具有明显地抑制作用,其半数抑瘤率(IC50)为7.19μmol/L,并有较好量效关系;流式细胞学检查发现SarcodoninG处理后的HeLa细胞出现凋亡现象、对P53蛋白表达影响与对照组比较有统计学意义(P<0.01);电镜观察发现SarcodoninG处理后的HeLa细胞的胞核染色质浓缩,边集,核固缩及形成新月体,线粒体肿胀,空泡样变,胞浆出现大量空泡等凋亡的形态学改变。结论鳞盖肉齿菌的纯化物SarcodoninG能抑制体外培养的HeLa细胞的增殖,可能是通过调节HeLa细胞P53蛋白表达,诱导HeLa细胞的凋亡来实现其抗肿瘤增殖作用的。更多还原

【Abstract】 BACKGROUND&AIM: To study the anti-tumor effects of Sarcodonin G,extracted from Sarcodon scabrosus karst by dichlorolomethane on HeLa cell in vitro and explore the possible mechanisms. MATERIAL AND METHODS: The effect of Sarcodonin G on HeLa cell line was determined by MTT assay in vitro. The apoptosis induced by Sarcodonin G was studied by FCM. The morphological changes of HeLa cells were assessed by transmission electron microscope. RESULTS:Sarcodonin G dose dependently inhibited the proliferation of cultured HeLa cells,the IC50 was 7.19 μmol/L. Compared with the control group,the apoptosis rate and the expression of P53 in HeLa cells were markedly higher in the three Sarcodonin G group(P<0.01). The morphological changes of HeLa cells incubated with Sarcodonin G showed chromatin condensation,marginal and nuclear fragmentation. CONCLUSION: Sarcodonin G could inhibit the proliferation of HeLa cells in vitro. Its anti-tumor effect might be associated with up-regulating the expression of P53 and inducing apoptosis.更多还原