【摘要】 背景与目的利用基因芯片技术研究肺腺癌组织及其转移淋巴结组织基因表达差异。材料与方法分别抽取3例肺腺癌组织、转移淋巴结组织的总RNA,通过逆转录合成cDNA,以两种荧光cy5和cy3标记,制备cDNA探针,并与含有1152个人类全长基因的cDNA基因芯片进行杂交。以ScanArray4000荧光扫描仪扫描芯片上两种荧光信号,用计算机处理和分析。结果3例肺腺癌组织和转移淋巴结组织标本中,8个基因有共同表达差异,其中上调基因5个,下调基因3个。结论肺腺癌组织和转移淋巴结组织之间存在着基因表达差异,两者比较共表达差异的8个基因可能与肺腺癌的发展、转移有关。更多还原

【Abstract】 BACKGROUND & AIM: To investigate the differences of gene expression profile among the pulmonary adenocarcinoma and metastatic lymph node tissues by means of cDNA microarray. MATERIAL AND METHODS: Total RNA was extracted from pulmonary adenocarcinoma and metastatic lymph node tissues in order to get purified mRNA which was retro-transcribed into cDNA.As the probes,the cDNA was labeled with Cy5and Cy3fluorescence,then hybridized with the whole human gene chip.The signal image was obtained by Scan Array4000fluorescence scanner and analyzed with computer software. RESULTS: 8genes in3subjects with pulmonary adenocarcinoma and metastatic lymph node tissues were identified with common differences in gene expression level,including5up-regulated and3down-regulated genes. CONCLUSION: The expression levels of8genes found both in pulmonary adenocarcinoma and metastatic lymph node tissues were probably related to the development and metastasis of lung cancer.更多还原