【摘要】 背景与目的深入探讨睾丸镉毒性机制。材料与方法应用cDNA微阵列分析和实时定量PCR技术对低剂量镉处理后大鼠睾丸组织基因表达情况进行分析。结果UDP-葡萄糖醛酸转移酶、血红素加氧酶、错配修复蛋白、T-激肽原和cal megin等基因与镉毒性相关,但作为产能的细胞器,线粒体呼吸链并未受到低剂量镉影响。另显示维生素C对镉毒性有明显的缓解作用。结论镉诱导毒性和致癌作用的影响可能涉及能量代谢、防御保护、DNA修复多个方面;维生素C能够明显降低镉对大鼠睾丸的毒性作用。更多还原

【Abstract】 BACKGROUND&AIM: To identify genes involved in cadmium-induced testicular toxicity. MATERIAL AND METHODS: We used cDNA microarray and real-time quantitative polymerase chain reaction(real-time PCR)technique to analyze the genes expression of testes in rats treated with low cadmium(4μmol/kg). RESULTS: Our studies for the first time demonstrated that expression of T-kininogen,calmegin,UDP-glucuronyl transferase,heme oxygenase and mismatch repair protein gene were associated with cadmium toxicity.However,as the organelles that produced energy,mitochondrion was not affected in the respiratory chain at low dose cadmium.In addition,vitamin C at the concentration of400mg/kg obviously attenuated cadmium-induced toxicity in testes of rats. CONCLUSION: The cadmium-reduced toxicity carcinogenic effects involved energy metabolism,defense mechanisms and DNA repair of the cell.Vitamin C might play essential roles in protecting testes of rats exposed to cadmium.更多还原