【摘要】 背景与目的白桦酯醇是一种重要的三萜类生物活性分子,本文从白桦树皮中提取获得白桦酯醇纯品,然后研究该生物活性分子抑制食管癌细胞的活性。材料与方法取一定量的白桦树皮干粉,通过乙醇回流,减压浓缩和甲醇-氯仿重结晶等步骤获得白桦酯醇纯品。用不同剂量的白桦酯醇作用于EC109食管癌细胞48h,噻唑蓝(Methylthiazoletetrazolium,MTT)法检测细胞抑制率。结果白桦酯醇作用于食管癌细胞EC109后,EC109细胞形态发生明显改变,细胞抑制率随白桦酯醇浓度的增高而增高,呈现剂量依赖关系。结论白桦酯醇对食管癌细胞EC109有明显抑制活性。更多还原

【Abstract】 BACKGROUND&AIM: Betulin is an important triterpene.We investigated the antitumor activity of betulin,extracted from the bark of silver birch(Betula platyphylla Suk). MATERIAL AND METHODS: Betulin was isolated from dry powder of the bark of silver birch(Betula platyphylla Suk)by using ethanol circumfluence,decompress concentration and methanol-chloroform recrystallization.The inhibition rate on EC109cell growth was detected by MTT(Methylthiazoletetrazolium)method,after the oesophageal cancer EC109cells have been treated for48h with different doses of betulin. RESULTS: With the increasing doses of betulin,the inhibition rate of EC109cell growth was increased,and their morphological characteristics were changed significamtly.The inhibition rate showed dose-dependent relation. CONCLUSION: Betulin had potent inhibiting effects on EC109cells growth in vitro.更多还原