【摘要】 目的:探讨加味四君子汤(简称中药)对实验性脾虚大鼠胃肠肽及回肠电-机械活动的影响。方法:采用放射免疫分析(RIA)方法对实验性脾虚证大鼠回肠始段、血液 P 物质(SP)、血管活性肠肽(VIP)进行测定,并用双银球电极-应变片记录了实验性脾虚证大鼠回肠始段的电-机械活动。结果:脾虚组 SP 含量显著升高(P<0.01),VIP 含量显著降低(回肠 P<0.05,血液 P<0.01),慢波和运动平均频率增高(P<0.05),运动振幅有增高趋势,快波明显增多;与脾虚组比较,自然恢复组 SP 含量明显恢复(回肠 P<0.01,血液 P<0.01),但未至正常;中药组 SP 含量降低(P<0.01),血液 VIP 含量显著回升(P<0.05),并接近正常,运动振幅显著下降(P<0.05),快波显著减少,其他指标无显著恢复。结论:脾虚证大鼠回肠始段 SP、VIP 含量改变可能与脾虚证大鼠回肠电-机械活动紊乱有关,中药对实验性脾虚证大鼠回肠始段胃肠肽的分泌及电-机械活动异常有一定的调理作用。更多还原

【Abstract】 Objective:To study the effect of Jiawei Sijunzi Decoction(JSD)on gut peptides and electric mechanical activity of ileum in rats with Spleen Deficiency Syndrome(SDS).Methods:Radioimmunoassay(RIA)was used to measure the substance P(SP),vasoactive intestinal peptides(VIP),and double silver ball electrode stress plate was used to record the electric mechanical activity of the beginning segment of ileum.Results:In the rats with SDS,SP content raised significantly(P<0.01),VIP content lowered significantly(P< 0.05 for ileal content and P<0.01 for blood content),slow wave frequency and average movement frequency increased(P<0.05), with quick wave increased and a trend of movement amplitude heightening.As compared with the parameters in SDS rats,the SP con- tent of natural recovered rats restored significantly(P<0.01)but not reaching normal range,while in the JSD treatment group,SP level restored(P<0.01)and VIP content in blood increased to approach normal range(P<0.05),the movement amplitude lowered and quick wave decreased significantly,but with no significant recovery in other parameters.Conclusion:Changes of SP and VIP content in the beginning segment of ileum of rats with SDS might be related with its electric mechanical activity disturbance.JSD has certain effects in regulating gut peptides secretion and adjusting the abnormal electric mechanical activity.更多还原