【摘要】 目的 :研究当归、丹参和川芎嗪注射液 (简称中药 )对腹膜透析 (PD)腹腔巨噬细胞 (MC)功能的影响。方法 :(1)将MC置于含当归、丹参和川芎嗪的腹膜透析液 (CDS)的培养液中培养 2 4h ,测定 3个中药组、CDS组和对照组MC的一氧化氮 (NO)含量和对四甲基偶氮唑盐 (MTT)还原能力。 (2 )MC分别置于含 2、10、10 0 μg/ml的 3个中药的CDS中培养 2 4h ,观察不同浓度中药对MC吞噬能力、NO含量和MTT还原能力的影响 ,对照组为等量培养液代替CDS。结果 :CDS组MC的NO含量和MTT还原能力均明显低于对照组 (P <0 0 1) ;与CDS组比较 ,川芎嗪组的MCNO含量和 3个中药组MCMTT还原能力均有明显提高 (P <0 0 1) ,并随着培养液中的中药浓度的提高 ,3个中药组MC吞噬能力和NO含量均有显著增加。结论 :在CDS中加中药能改善腹膜腔MC的防御功能 ,降低腹膜炎的发生率 ,对提高PD疗效有重要意义。更多还原

【Abstract】 Objective: To study the effect of Angelica sinensis, Salvia miltiorrhiza and Ligustrazine on function of peritoneal macrophages during peritoneal dialysis. Methods: Peritoneal macrophages of mice were cultured in culture medium (control), peritoneal dialysate (PD), drugs contained PD containing Angelica, Salvia and Ligustrazine combined (PD ASL) or separated (PD A, PD S, PD L) with concentration of 2μg/ml, 10μg/ml and 100μg/ml, separately for 24 hrs. The nitric oxide (NO) content, methyl thiazolyl tetrazolium (MTT) reducing capacity (MTT RC) and phagocytosis capacity of macrophages were determined and compared. Results: NO content and MTT RC of macrophages cultured in PD group were significantly lower than those of the control (P<0 01), as compared with those in drug contained PD groups, the NO content in the PD L group and the MTT RC in the PD ASL group were higher significantly (P<0 01). The phagocytosis capacity and NO content in the PD ASL group were raised along with the increased concentration of drug in PD. Conclusion: Administering Chinese herbal medicine during peritoneal dialysis has important significance in improving the defense function of peritoneal macrophages, reducing the incidence of peritonitis and enhancing the therapeutic effect of peritoneal dialysis.更多还原