【摘要】 目的　研究在细胞周期G2 /M期特异性的Survivin基因启动子在多型性胶质母细胞瘤 (glioblastomamultiform ,以下简称胶质瘤 )细胞株T98G中的活性 ,为该启动子在胶质瘤靶向性基因治疗中的应用提供依据。方法　用RT PCR法检测Survivin基因在胶质瘤细胞株T98G及正常肝中的表达 ,用转染荧光素酶报告基因的方法 ,比较不同长度的Survivin基因启动子在非同步化和阻断于G1期细胞中的活性。结果　Survivin基因在T98G胶质瘤细胞中表达 ,而在正常肝细胞中不表达。Survivin基因启动子在非同步化细胞中的活性大约是G1期细胞中的 11倍。结论　Survivin启动子在转录水平上可以选择性地使报告基因在胶质瘤细胞中表达 ,这使它成为一个在胶质瘤基因治疗中有潜力的启动子。更多还原

【Abstract】 Objective To Study the activity of the survivin gene promoter in glioblastoma multiform cell line T98G and evaluate the possible application of this promoter in glioblastoma gene therapy. Methods Use RT PCR to detect the expression of survivin gene in the T98G cell line and normal adult liver. By luciferase reporter gene, the activity of survivin gene promoter with two truncated forms were compared in non synchronized T98G cells and G 1 arrested T98G cells. Results Survivin gene is expressed in T98G cell line but not expressed in normal adult liver tissue. The activity of survivin promoter in non synchronized cells is 11 times as great as that in G 1 arrested cells. Conclusion In transcription level, survivin promoter can selectively activate the reporter gene in glioblastoma, and this makes it a potential candidate promoter in the gene therapy of glioblastoma.更多还原