【摘要】 研究了磷脂酶A2 在水相中水解浓缩磷脂的最佳工艺条件 :底物浓度 10 % ,反应温度5 5℃ ,pH值 8 5 ,Ca2 +浓度 0 2mol/L。在最佳条件下得到产品经HPLC检测 ,PC的转化率为92 7% ,并研究了水解速度与时间的关系。水解得到的溶血磷脂产品的HLB大于 8。更多还原

【Abstract】 The hydrolysis of lecithin by phospholipase A 2 in water was studied, then the optimum conditions of reaction was investigated by the orthogonal experiment, which were substrate concentration 10 percent, reaction temperature 55℃ pH 8.5, Ca 2+ concentration 0.2 mol/l . The convertion of phosphatidycholine was 92.7% under this condition determined by the HPLC. The relationship of rate of hydrolysis and reaction time was also examined. The HLB of lysophospholipids product was over 8.更多还原