【摘要】 强脊汤对强直性脊柱炎有确切的疗效 ,为进一步探讨该方药对机体免疫功能的影响 ,以TNF、IL 2为指标进行了实验观察。实验 Ⅰ 选用 30只SD大白鼠 ,将Freunds氏完全佐剂注射于大鼠左后足掌皮下造成佐剂性关节炎模型 ,随机分为强脊汤组、消炎痛组、对照组 ,分别灌胃给药 2 0天后下腔静脉取血 ,采用放免法测定TNF。实验Ⅱ 选用 6 0只昆明种小白鼠 ,采用腹腔注射AZP和Cy法造成免疫超常模型和免疫抑制模型 ,随机分为强脊汤Ⅰ 组、消炎痛Ⅰ 组、对照 Ⅰ 组 ,强脊汤 Ⅱ 组、消炎痛 Ⅱ 组、对照 Ⅱ 组 ,分别灌药 7天后挖眼取血 ,采用放免法测定IL 2。结果显示 ,该方药对佐剂性关节炎模型大鼠体内TNF水平具有明显的降低作用 ,并可双向调节免疫异常小鼠体内的IL 2水平。表明该方药对改善免疫紊乱状态具有确切可靠的疗效。更多还原

【Abstract】 Objective: To explore the effect of "Qiangjitang" on immunologic function, TNF and IL-2 were observed. Methods: Thirty SD rats were injected in left and behind plantor skin by Freunds adjuvant and made into adjuvant arthritis model, then divided into Qiangjitang group, Xiaoyantong group and control group randomly. After feeding 20 days the rats were taken blood from inferior vena cava and TNF was measured by radioimmunoassay. Sixty Kunming’ mice were injected AZP and Cy by intraperitoneal injection, and made into immunoenhancement model and immunosuppressant model, then divided into Qiangjitang groupⅠ, Xiaoyantong groupⅠ,control groupⅠ, Qiangjitang groupⅡ, Xiaoyantong groupⅡ and control groupⅡ randomly. After feeding 7 days the mice were taken blood from eyes and IL-2 was measured by radioimmunoassay. Results: It showed that "Qiangjitang" can obviously decrease TNF of adjuvant arthritis rats and adjust IL-2 of dysimmunity mice bidirectionally. Conclusion: It was suggested that "Qiangjitang" has reliable curative effect to dysimmunity.更多还原