【摘要】 目的克隆血糖调节基因。方法按本室以往建立的方法制备大鼠血糖自动调节模型,以灌输生理盐水为对照;取其骨骼肌,采用差异显示技术(DDRT-PCR),获得差异表达基因片段,经狭缝杂交和Northern杂交分析,排除假阳性片段,确定真正的差异表达序列标签(EST),并以其为探针筛选大鼠骨骼肌cDNA文库。结果获得一个新的全长cDNA,命名为Fang-2,GenBank收录号为AF399874。经采用Blast软件(NCBI)分析显示,Fang-2是人类troponinT在大鼠中的同源物,其同源性为78%,表明该家族蛋白具有保守性。在高浓度的葡萄糖刺激大鼠后,和对照大鼠相比,Fang-2的表达下降。结论从大鼠骨骼肌中克隆到一个参与血糖调节的新基因,该基因可能通过其表达下调控制或部分控制某些目前未知的机制而达到调节血糖水平的作用。更多还原

【Abstract】 or Objective To clone a novel gene relative to blood glucose regulation.Methods Rat modes of autonomous regulation of blood glucose was made by intra jugular vein to right atrium injection of high concentration of glucose solution,and the control rats were injected with0.9%NaCl both before skeletal muscles were separated for gene analysis.The differentially expressed fragments were identified by differential display technology(DDRT-PCR).After slot blot and Northern blot analysis,the artificial positive fragments were excluded and the true EST (expression sequence tag)differentially expressed was obtained.These positive EST were used as probes to screen cDNA library of rat skeletal muscle.Results A novel full-length cDNA,named as Fang-2was obtained.GenBank Accession No.was AF399874.Fang-2was found rat homologue of human troponin T by blast software(NCBI).It shared78%identical nucleotides,which showed the family proteins were conservative.After high concentra-tion of glucose stimulation of rats,the expression of Fang-2was down-regulated.Conclusions A novel gene relative to blood glucose regulation was cloned from rat skeletal muscle.The gene can regulate blood glucose level by effect certain mechanisms unknown yet with down-regulation expression.更多还原