【摘要】 目的 为了观察三氯乙烯的致突变作用。方法 本文采用剂量为50 000、10 000、2 000、400、80μg/皿的三氯乙烯对鼠伤寒沙门氏菌株TA97、TA98、TA100、TA102进行染毒,并于37℃培养48 h后观察其菌落数。结果 菌株TA97、TA98、TA102不论是否加入S₉,其菌落数没有比空白对照组增加1倍以上。而TA100菌落数比空白对照组增加一倍以上,三氯乙烯在S₉存在的情况下导致鼠伤寒沙门氏菌株TA100增加的数量比没有S₉存在的情况下多。结论 三氯乙烯是能导致菌株TA100发生突变的致突变剂（碱基对置换）。三氯乙烯的致突变作用可能部分是由于三氯乙烯经过代谢成中间活性产物而起作用的。更多还原

【Abstract】 Objective To investigate the effect of trichloroethylene Mutation. Methods Trichloroethylene of different doses (50 000, 10 000, 2 000, 400, 80μg/ ) were used to make typhic Salmonella lines{TA97, TA98,TA100,TA102) infected. Colonies in every groups were observed and counted respectively after being cultivated for 48 hrs at 37℃ . Results The number of TA100 colonies with S9 were as twice as which in control group, while TA97, TA98, TA102 were not. The increase of the number of typhic Salmonella lines TA100 caused by Trichloroethylene with the existence of S9 is more that without it. Conclusion Trichloroethylene can be metabolized into intermediate and has mutagenicity on typhic Salmonella TA100.更多还原