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培养条件下肌腱中心区域腱细胞的增殖能力
PROLIFERATIVE ABILITY OF THE CENTRAL AREA TENOCYTES OF FLEXOR TENDONS IN VITRO
【摘要】 目的 研究肌腱损伤后腱中心区域组织的愈合能力。方法 选用 8只白色纯种来亨鸡 ,在无菌条件下切取双侧最长趾 区趾深屈肌腱 ,切成 4 mm长的肌腱段 ,分为两组 ,每组 12条。实验组 :切除腱外膜和外膜下腱组织的肌腱中心区域组织 ;对照组 :仅剥除腱外膜组织的肌腱段。将两组肌腱组织进行体外培养 ,于培养第 9、18及 2 7天取标本进行大体观察及组织学检查。另取 4条肌腱作正常对照组 ,直接进行大体观察和组织学检查。结果 各时间点的腱细胞数 ,对照组均明显少于实验组及培养前 ,有统计学意义 (P<0 .0 1) ;实验组比培养前均明显增多 ,有统计学意义 (P<0 .0 1) ;但培养第 18天和 2 7天时 ,实验组腱细胞数较培养第 9天少 (P<0 .0 1)。结论 屈肌腱损伤后腱中心区域组织有良好的愈合能力。
【Abstract】 Objective To study the healing ability of the central area tissue of flexor tendons after injury. Methods Tendons of flexor digitorum profundus of the long toes from 8 white Leghorn hens were harvested in zone II. Tissues were cut in 4 mm segments and divided into the experimental group(the central area tissue of flexor tendons) and the control group(the tendon segments without epitenon). There were 12 tendon segments cultured in each group. Specimens were obtained and examined under light microscope on the 9th, 18th and 27th days after culture, respectively. Another 4 tendons were used as normal control, and they were directly examined under light microscope. Results The number of tenocytes was significantly less in the control group than in the experimental group and the uncultured state ( P< 0.01); the number of tenocytes was significantly higher in the experimental group than in the uncultured state ( P< 0.01). The number of tenocytes of the experimental group were higher on the 9th day than on the 18th and 27th days after culture( P< 0.01). Conclusion The central area tissue of flexor tendons has favorable healing ability after injury.
- 【文献出处】 中国修复重建外科杂志 ,Chinese Journal of Reparative and Reconstructive Surgery , 编辑部邮箱 ,2002年04期
- 【分类号】R686.4-33
- 【下载频次】44