【摘要】 目的　探讨血清一氧化氮 (nitric oxide,NO)水平、超氧化物歧化酶 (superoxide dismutase,SOD)活性在围手术期心肌再灌注损伤中的变化及临床意义。方法　心脏手术组 2 5例 ,分别在术前 1d,主动脉开放后 10 min,术后 1d和 7d采血 ,化学比色法测定血清 SOD,酶标法测定血清 NO。结果　心脏手术组术前 NO为 47μmol/ L± 10μmol/ L ,再灌注 10 min为 80μmol/ L± 10μmol/ L (P<0 .0 1) ,术后 1d,6 7μmol/ L± 12μmol/ L (P>0 .0 5 ) ,术后 7d,49μmol/ L±14μmol/ L (P>0 .0 5 ) ,术前 SOD为 144 Nu/ m± 2 5 Nu/ ml,再灌注 10 min为 114Nu/ m L± 2 4Nu/ m L (P<0 .0 1) ,术后 1d,141Nu/ m L± 2 4Nu/ m L (P>0 .0 5 ) ,术后 7d,144 Nu/ m L± 2 4Nu/ m L (P>0 .0 5 )。血清 NO与 SOD水平呈负相关 (r=-0 .495 ,P<0 .0 5 )。结论　血清 NO水平增高 ,氧自由基大量产生 ,SOD活性降低是造成心肌再灌损伤的重要原因更多还原

【Abstract】 Objective To study the changes of serum NO level and SOD activity during perisurgical myocardial reperfusion injury and their clinical significance.Methods SOD activity and No level in CPB group(n=25) were examined quantitatively with chemical colorimetry and enzyme labeled method respectively, and blood samples were collected 1 day before operation(A),10 minutes after opening of aorta CPB(B),1st(C) and 7th(D) day after operation respectively.Results As for NO,there was remarkable significance between A and B (P<0.01),while no significance was found either between A and C or between A and D.As for SOD,the results were the same.There was a negative correlation between NO level and SOD activity(r=-0.495,P<0.05).Conclusions Increased serum NO level,production of a deal of oxide free radicals and decreased SOD activity were the main reasons for myocardial reperfusion injury.更多还原