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一个小麦丝氨酸-苏氨酸蛋白激酶基因的克隆和分析(英文)
Molecular Cloning and Characterization of a Serine/Threonine Protein Kinase Gene from Triticum aestivum
【摘要】 用mRNA差异显示技术在含有抗白粉病基因Pm2 1的小麦 (TriticumaestivumL .)_簇毛麦 (Haynaldiavillosa)6VS/ 6AL易位系 92R137中分离与抗白粉病相关的基因 ,获得一个命名为TaPK1的全长cDNA克隆。序列分析表明 ,它与大豆 (Glycinemax (L .)Merr.)蛋白激酶基因GmPK6高度同源。经推测 ,TaPK1编码 416个氨基酸的多肽 ,属丝氨酸_苏氨酸蛋白激酶家族 ,并具酪氨酸激酶特性。TaPK1是从小麦中分离的新基因。
【Abstract】 To isolate genes related to resistance to Erysiphe graminis DC. ex Merat f. sp. tritici Em. Marchal in wheat (Triticum aestivum L.), differential display analysis was conducted for mRNA extracted from the seedlings of the wheat_Haynaldia villosa 6VS/6AL translocation line 92R137 that contains the powdery mildew resistance gene Pm21. A full_length cDNA named TaPK1 was isolated. BLAST analysis revealed that it was significantly homologous to Glycine max (L.) Merr. protein kinase (GmPK6) cDNA. TaPK1 encodes a 416 amino acid long polypeptide, which belongs to serine/threonine protein kinase family, also has tyrosine kinase specificity. TaPK1 is a novel protein kinase from wheat.
- 【文献出处】 Acta Botanica Sinica ,植物学报(英文版) , 编辑部邮箱 ,2002年03期
- 【分类号】Q943.2
- 【被引频次】23
- 【下载频次】380