【摘要】 采用柠檬酸三钠还原法制备胶体金颗粒 ,标记烟草环斑病毒的抗体 ,制成免疫层析检测试纸条。检测粗提纯病毒的灵敏度为 1 0 0 0ng/ml,病汁液稀释 1 0 0 0倍后仍可快速检出。对大豆病种子、烟草冻干病叶等不同材料进行检测也有良好的效果 ,1～ 2min即可出现结果。用 9种不同的病毒进行测试 ,未出现非特异性反应。更多还原

【Abstract】 Gold immunochromatography assay was developed by coupling colloidal golds with rabbit polyclonal antibody against tobacco ringspot virus.The sensitivity of GICA was 1000 ng/ml for detecting partially purified tobacco ringspot virus.Extract from infected tobacco leaf was detected to a dilution of 10 -2 .Satisfied results were obtained within 1～2 minutes by testing infected soybean seed or freeze dried tobacco leaf.Non specific reaction was not observed for testing other 9 viruses.更多还原