【摘要】 目的探讨一组正反互补单克隆抗体对胃癌患者腹腔冲洗液中脱落癌细胞检测的应用价值。方法应用低分子量细胞角蛋白(CKLMW)、癌胚抗原(CEA)、间皮细胞(MC)及波形蛋白(Vim)4种标志物,对67例胃癌患者腹腔冲洗液中的癌细胞成分进行免疫细胞化学(ICC)检测,并与常规细胞病理学(CP)检测结果比较。结果ICC阳性检出率(86.2%)明显优于CP阳性诊断率(58.6%)(P<0.05);ICC阳性检出率随侵袭深度及病期进展而增加;CKLMW、CEA、MC及Vim4种抗体的可用度分别为64.6%、71.6%、77.9%、41.8%。结论联合应用CKLMW、CEA及MC对于检测胃癌腹腔冲洗液中脱落癌细胞具有重要的临床价值,而Vim缺乏诊断意义。更多还原

【Abstract】 Objective To evaluate the applied value of a pan el of help each other monoclonal antibodies for detecting exfoliative gastric c ancer cells in peritoneal washing.Methods As immunocytochemical(ICC)methods,four markers including low-molecular-weight cytokeratin(CK LMW ),carcinoembryonic antigen(CEA),mesothelial cell(MC)and vimentin(Vim)were used to immunostain the cells in peritoneal washing from 67patients with gastric cancer.Results ICC was superior to cytopathology(CP)for detecting exfoliative gastric cancer cells with the positi ve rate 86.2%and 58.6%respectively.Expression levels of these four markers increased in accordance with the depth of invasion and the progre ssion of disease stage.Reliabiliti es of CK LMW ,CEA,MC and Vim were 64.6%,71.6%,77.9%and 41.8%respectively.Conclusion Combination of immunocytochemial use with a panel of CK LMW ,CEA and MC antibodies except Vim possesses an important applied value for detec ting exfoliative gastric cancer cells in peritoneal washing.更多还原