【摘要】 小麦 (Triticum aestivum)品种洛夫林 10和叶锈菌小种 36 6组成不亲和组合 ,小麦叶片发生过敏性坏死反应 (HR)是小麦抵抗叶锈菌侵染的重要因素。在接种前给小麦叶片分别预注射微管解聚药物磺草硝 (oryzalin)和微丝解聚药物细胞松弛素 D(cytochalasin D,CD) ,结果表明 2种药物注射使得寄主因叶锈菌侵染诱导的细胞过敏性坏死数目明显减少 ,并且注射药物的浓度越大 ,寄主细胞发生 HR的数量越少。说明肌动蛋白和微管蛋白的聚合状态是诱发小麦叶片发生 HR防卫反应所必需的 ,细胞骨架在小麦抵抗叶锈菌侵染过程中可能起着重要作用。更多还原

【Abstract】 Two kinds of cytoskeleton depolymerizing reagents, cytochalasin D(CD) and oryzalin, were separately injected into the primary leaves of wheat ( Triticum aestivum) (cv. Lovrin 10) seedlings immediately before the leaves were inoculated with an incompatible race 366 of leaf rust ( Puccinia recondita f. sp. tritici ) to study their effects on the hypersensitive reaction (HR), an important defense response of host cells in the incompatible interaction of wheat leaf rust . The results showed that both the reagents could obviously reduce the number of the necrotic cells. The higher the concentration, the fewer the necrotic cells. These results indicated that the polymerized filamentous state of actin or tubulin is necessary for host plant to express HR, and strongly suggest that microfilaments and microtubules play an important role for wheat to block the infection of leaf rust fungus.更多还原