【摘要】 用荧光检测的高效液相色谱法同时检测鸡组织中噁喹酸、氟甲喹的残留量。用1 mol/L磷酸二氢钾溶液作为提取液,过C18固相萃取柱净化、浓缩。HPLC条件:荧光检测器,激发波长325 nm;发射波长369 nm。流动相:0.02 mol/L磷酸-乙腈-四氢呋喃(69∶16∶15)。噁喹酸、氟甲喹标准曲线线性范围在10~500 ng/mL。对不同组织的3种浓度进行回收率测定,其回收率在61%~92%之间,变异系数在15%以内。噁喹酸、氟甲喹的检测灵敏度为20 ng/g。更多还原

【Abstract】 The method of high-performance liquid chromatography (HPLC) with fluorescence detection wasused for the simultaneous determination of oxolinic acid and flumequine residues in chicken tissues. The drugswere extracted with 0.1 mol/L aqueous potassium dihydrogenphosphate, purified and concentrated by passingthrough Bond Elut C18 solid-phase extraction cartridges. The HPLC separation was carried out on a Luna C18 5μmcolumn (150 mm×4.6 mm i.d.) with 0.02 mol/L phosphoric acid - acetonitrile - tetrahydrofuran (69∶16∶15)as the mobile phase. A fluorescence detector was used at an excitation wavelength of 325 nm and an emissionwavelength of 369 nm. The calibration graphs were linear between 10 ng/mL and 500 ng/mL for oxolinic acid andflumequine. Average recoveries of the drugs from chicken tissues fortified at a level of 20~300 ng/g were 61%~92%.Coeffcients of variation were 15%, and the detection limits for oxolinic acid and flumequine were 20 ng/g.更多还原