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供体PGCs在鸡-鸭嵌合体胚胎性腺外的发育
Extragonadal Development of Donor PGCs in Chicken-Duck Chimeric Embryos
【摘要】 通过微注射法将鸡原始生殖细胞 (PGCs)注入到鸭的胚盘下腔中 ,用鸡 W染色体 DNA探针通过原位杂交的方法对供体 PGCs在嵌合体胚胎性腺外的发育作了研究。结果表明 ,所取的 4 8枚胚胎中 ,有 18.8%的脑内和 2 3.9%的神经管周围出现了阳性信号
【Abstract】 Chicken PGCs from a line of White Leghorns were microinjected into Maya duck blastoderms at stage Ⅹ.Extragonadal development of donor PGCs in chicken duck chimeric embryos were studied by in situ hybridization with W chromosome specific DNA probes.In approximately 18 8% and 23 9%,respectively,of species from 48 chimeric embrys the chicken PGCs migrated into the brains and the neural tubes.
【关键词】 鸡-鸭嵌合体;
供体PGCs;
性腺外的发育;
【Key words】 chicken duck chimera; donor PGCs; extragonadal development;
【Key words】 chicken duck chimera; donor PGCs; extragonadal development;
- 【文献出处】 中国兽医学报 ,Chinese Journal of Veterinary Science , 编辑部邮箱 ,2002年04期
- 【分类号】S831.1
- 【被引频次】4
- 【下载频次】99