【摘要】 对 75只波尔山羊超排处理 ,共获 A、B级胚胎 776枚 ,平均 (10 .35± 6 .4 5 )枚 /只 ,鲜胚移植受体 36 7只 ,移植产羔率达 5 5 .6 %。应用 CIDR和 PMSG同期发情处理受体山羊 5 6 0只 ,5 4 9只达到同期发情 ,32 h内同期率为 98.0 4 %。囊胚和扩张囊胚在发情后 7d的移植产羔率分别为 6 2 .2 2 % (84 /135 )和 5 8.2 6 % (6 7/115 ) ,显著高于桑椹胚 (43.18% )和孵化囊胚 (5 1.72 % ) (P<0 .0 1) ,而囊胚和扩张囊胚间、桑椹胚和孵化囊胚间差异不显著 (P>0 .0 5 )。受体山羊的黄体数量直接影响到移植产羔结果 ,黄体数≥ 2的受体山羊移植产羔率显著高于黄体数为 1的受体山羊移植产羔率 (P<0 .0 1) ,分别为 6 4 .83% (94 /14 5 )和 4 9.5 5 % (110 /2 2 2 )。PMSG注射时间 (在取 CIDR前 2 d或当天注射 )和剂量对受体羊的黄体数和移植产羔率均无显著影响 (P>0 .0 5 )更多还原

【Abstract】 Embryo transfer is one of the biotechnologies in the field of livestock breeding.A total of 75 Boer goats were treated for superovulation and 766 embryos of grade A and grade B were obtained.The average number of transferable embryos per goat was 10 35±6 45.Part of the embryos were transferred to 367 recipients and the kidding rate was 55 6%(204/367).Treated with CIDR and PMSG for estrus synchronization,549 of recipients( n =560) showed estrus and the synchronization rate within 32 h was 98 04%.The kidding rats of blastocysts,expanded blastocysts,morulae,and hatched blastocysts transplanted at 7 d after estrus were 62 22%(84/135),58 26(67/135),43 18%(38/88),and 51 72%(15/29),respectively.The kidding rates of blastocysts and expended blastocysts were significantly higher than those of morulae and hatched blastocysts( P <0 01).The number of corpus luteum in recipients significantly affects the kidding rate.The kidding rate of recipients with 2 or more corpus lutea was significantly higher than that of with 1 corpus luteum(64 83%(94/145) vs 49 55%(110/222), P <0 01).The number of corpus luteum in recipients and kidding rate were not different when PMSG was injected on the day CIDR removed or 2 days before(54 82%(108/97) vs 55 84%(86/154), P >0 05).The dosage of PMSG(150 IU or 200 IU) for estrus synchronization showed no effects on the number of corpus luteum in recipients and the kidding rate( P >0 05).The fertility of the flock could be increased by 6 8 times with application of ET project in Boer goats.更多还原